Nesi-Khonsu was the wife of the high priest Pennedjem the second and the daughter of his elder brother Smendes the second, who preceded Pennedjem in his post during the Twenty-First Dynasty.

Nesi-Khonsu died before her husband when she was fairly young. Her mummy is one of the best examples of Twenty-First Dynasty embalming; the entire body was very finely packed and molded, the eyes are inlaid with stone and the arms are fully extended with the palms turned into the thighs.

Flowers were wrapped round the big toe of each foot as well as round the left foot and the ankles.

Nesi-Khonsu is distinguished from the other female mummies by her hairstyle, which has two side braids like those worn by Egyptian peasant girls.